Engineering a Floxuridine-integrated RNA Prism as Precise Nanomedicine for Drug Delivery

Herein, we report a geometrically well-defined prism assembled by two floxuridine(F) integrated RNA strands as a precise nanomedicine for chemodrug delivery. Owing to the similarity between chemotherapeutic F and uridine(U), all uridines in the component RNA strands are replaced by F during their in vitro transcription syntheses. By specifically designing their sequences, F-containing S-shape tiles with the single-stranded loops are constructed and then further assemble into the RNA prism through T-junction interactions and sticky-end cohesions. The present study demonstrates that the F-integrated RNA prism can serve as an efficient platform for chemodrug delivery and anticancer treatment. We believe that the study would help the future development of RNA-based nanomedicine in various applications.