Antimicrobial Effect of Titanium Oxide (Tio2) Nano Particles in Completely Edentulous Patients. A Randomized Clinical Trial

Objective: to investigate the effect of addition different concentration of TiO2 nanoparticles on acrylic resin polymethyl methacrylate to improve the anti-microbial properties of denture base material Subjects and Methods: 24 completely edentulous patients were selected to be enrolled in of the following groups: Group I: 8 patients were rehabilitated with complete dentures had no nanoparticle (Control group),Group II: 8 patients were rehabilitated with complete dentures had a 1% concentration of titanium oxide nanoparticles and Group III: 8 patients were rehabilitated with complete dentures had a 3% concentration of titanium oxide nanoparticles . Microbiological evaluation was done from the fitting surface of dentures after one week, two weeks, and one month of denture use. Results: Regarding staphylococcus aureus and candida albicans colonization, the result revealed that between one week, two weeks , and one month follow up periods for each group and between different groups for each follow up period, there was a statistically significant difference between all groups. Regarding staphylococcus epidermis colonization , there was negative colonization of staphylococcus epidermis . Conclusion: Addition of TiO2 nano particles with 1% and 3% concentrations to PMMA has a great anti-microbial effect with reduction of the colonization of S. aureus, S. epidermis and C. albicans than the conventional dentures