EXPERIMENTAL AND COMPUTATIONAL APPROACH OF DYNAMIC CELL SEEDING FOR TISSUE ENGINEERING

A functional tissue engineered scaffold requires an optimum cell seeding process. Proper cell density and spatial distribution in a 3D scaffold are essential to morphogenetic development of an engineered tissue [Li et al, 2001]. A high number of cells and an even cell distribution in a scaffold are associated with better culture results [Wendt et al, 2006]. Since human cells are often available in short supply, maximization of the cell seeding process is necessary. The aim of the present study was to propose a complete method capable of predicting the cell seeding process under dynamic conditions for regular scaffolds.