Antimicrobial Activity of Calix[4]pyrrole-entrenched Silver Nanoparticles and Its Application as Colorimetric and Spectrophotometric Sensing of L-Histidine

A calix[4]pyrrole tetrahydrazide functionalized silver nanoparticles (CPTPH-AgNPs) coupled with colorimetric findings were prepared. In the variety of essential amino acids, it detected the L-Histidine exclusively with an LOD of 6.1 μM and LOQ of 18.5 μM. The CPTPH-AgNPs were exhibited the SPR spectrum at 408nm, which further characterized with TEM, EDEX and SAED in that they show the monodispersed spherical morphology of AgNPs with 13±2nm size. The antimicrobial potential of CPTPH-AgNPs towards Gram-positive bacterial-Staphylococcus aureus, Bacillus subtilis, Gram-negative bacteria-Escherichia coli, Pseudomonas aeruginosa and fungi-Aspergillus niger was evaluated.; in which it is found to give reasonable response comparable to standard antibiotic drugs.