Effects of Medium Components Contents on Vitamin E Accumulation in Wheat Embryo Callus

With accumulation of secondary metabolites vitamin E as the objective, the effects of six components contents in the culture on vitamin E accumulation in wheat embryo callus was studied and the best combination of six components in medium was determined by test optimum. The results showed that the production of vitamin E in B5 medium was the highest at KNO32700mg/L, MgSO4·7H2O 125mg/L, NAA 6mg/L, CaCl2·2H2O 75mg/L, FeSO4·7H2O 13.9mg/L and sucrose 30.0×103mg/L. Vitamin E accumulation varied obviously with sucrose content in the medium, but the influence of magnesium sulfate was not significant.