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Two-Acceptor Cyanine-Based Fluorescent Indicator for NAD(P)H in Tumor Cell Models
- Source :
- ACS Sensors. 1:702-709
- Publication Year :
- 2016
- Publisher :
- American Chemical Society (ACS), 2016.
-
Abstract
- Sensing of intracellular NAD(P)H is important for numerous applications ranging from diagnostic assays to drug discovery. Up to now, investigation of NAD(P)H-dependent pathways in live cells has been restrained by the lack of efficient tools. Toward this goal, we developed a small molecule indicator, which allows both colorimetric and fluorescent NAD(P)H detection in biological samples. The design is based on a cyanine dye scaffold and utilizes a novel two-acceptor “turn-on” mechanism. Consequently, this indicator features unprecedented sensitivity and rapid response toward NAD(P)H at low micromolar levels under physiological conditions. First, we demonstrated the value of this reagent in a diagnostic assay of glucose, through the enzyme-coupling reaction of NAD(P)+-dependent glucose dehydrogenase (GDH). Second, we showed the utility of our indicator for NAD(P)H imaging in live cells. We confirmed its ability to reflect different NAD(P)H levels using the human colon cancer cell line deficient on mitochond...
- Subjects :
- Fluid Flow and Transfer Processes
010405 organic chemistry
Process Chemistry and Technology
Bioengineering
Nicotinamide adenine dinucleotide
Biology
010402 general chemistry
01 natural sciences
Molecular biology
Small molecule
3. Good health
0104 chemical sciences
Redox indicator
chemistry.chemical_compound
chemistry
Biochemistry
Glucose dehydrogenase
biology.protein
NAD+ kinase
Cyanine
Instrumentation
Intracellular
Alcohol dehydrogenase
Subjects
Details
- ISSN :
- 23793694
- Volume :
- 1
- Database :
- OpenAIRE
- Journal :
- ACS Sensors
- Accession number :
- edsair.doi...........aa3819f365068644e3d89fa960da5fc1