Recombinant DNA Techniques

The aim of this chapter is to introduce the procedures currently used to isolate and manipulate yeast genes. We restrict our discussion to those procedures that are, at least in part, peculiar to the yeast Saccharomyces cerevisiae. We do not deal with procedures that are common to all other organisms, such as cDNA bank construction, hybrid-selected or arrested translation systems, nucleic acid probe strategies, or radioimmune detection strategies, as these have been covered in a variety of general texts (e.g., Maniatis et al., 1982; Glover, 1985).