Effect of 1-deoxynojirimycin on the Replication of Baculoviruses, Bombyx Mori Nucleopolyhedrovirus and Autographa Californica Multiple Nucleopolyhedrovirus

Department of Life science, The University of Suwon, Hwaseong 445-743, Republic of Korea(Received 09 June 2011; Accepted 30 August 2011) 1-Deoxynojirimycin (DNJ) is an alkaloid that is foundat relatively high concentrations in mulberry leaf andtissues of the silkworm, Bombyx mori. DNJ is a wellknown inhibitor of α-glucosidase, an enzyme that isinvolved in the early stages of the N-linked glycopro-tein synthesis pathway. α-Glucosidase activity in thecell extract from B. mori-derived Bm5 cells showedapproximately 40-fold less sensitivity to DNJ than α-glucosidase activity in the cell extract from Spodopterafrugiperda-derived Sf9 cells. The replication of B. morinucleopolyhedrovirus (BmNPV) was not inhibitedwhen it was propagated in BmN cells that were grownin medium containing up to 10 mM DNJ. In contrast,the replication of Autographa californica multiple NPV(AcMNPV) was reduced by 67% when it was propa-gated in Sf9 cells that were grown in medium contain-ing 10 mM DNJ. The viability of Bm5 and Sf9 cellsthat were grown in medium containing up to 10 mMDNJ was not affected. Our results suggested that thereduced replication of AcMNPV was the result of thehigher sensitivity of α-glucosidase activity in Sf9 cellsto DNJ.Key words: 1-Deoxynojirimycin, Bombyx mori nucleo-polyhedrovirus, BmNPV, Baculovirus, α-Glucosidaseinhibitor