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INFLUENCE OF ILLUMINATION CONDITIONS ON THE MEASUREMENT OF OPTICAL TWEEZERS SYSTEM

Authors :
Daozhong Zhang
Chunxiang Liu
Yuqiang Jiang
Zhaoli Li
Bingying Cheng
Honglian Guo
Suotang Jia
Source :
International Journal of Modern Physics B. 20:659-666
Publication Year :
2006
Publisher :
World Scientific Pub Co Pte Lt, 2006.

Abstract

In an optical tweezers system, the output signal of a photodiode quadrant detector and the temperature in a sample cell are two key factors for the quantitative measurements of mechanical properties of living biological objects such as cells, organelles and macro-molecules. In order to enhance the output of a quadrant detector and effectively control the temperature in a sample cell, the dependence of the temperature in the sample cell and the output of the quadrant detector for different illumination conditions are studied. The results show that appropriate illumination conditions can ensure both nearly constant temperatures in the cell and the desired output signal, which provides for the possibility of high precision and damage-free analysis of living biological objects.

Details

ISSN :
17936578 and 02179792
Volume :
20
Database :
OpenAIRE
Journal :
International Journal of Modern Physics B
Accession number :
edsair.doi...........a87966066687f65d62cabae3827561ab
Full Text :
https://doi.org/10.1142/s0217979206033553