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Meiotic resumption of oocytes developed in vitro from mouse fetal gonads
- Source :
- Reproductive Immunology and Biology. 21:58-63
- Publication Year :
- 2006
- Publisher :
- Japan Society for Immunology of Reproduction, 2006.
-
Abstract
- To investigate the developmental competence in vitro of female germ cells in fetal gonads, gonadal ridges of 12.5 days post coitus (dpc) were organ-cultured for 18 days, and then oocyte-granulosa cell complexes (OGCs) were isolated from the cultures. Collected OGCs were further cultured for 11 days. The oocytes grown in vitro reached 64.1 ± 0.4 μm. Transmission electron microscopy analysis showed that oocytes grown in vitro had maintained connection with surrounding somatic cells. Some oocytes resumed meiosis by 1 μM okadaic acid or 10 ng/ml epidermal growth factor (EGF). The oocytes that underwent germinal vesicle breakdown reached 71.3 ± 0.5 μm. This study showed growth and meiotic resumption in vitro of oocytes through the support of ovarian tissue from fetal gonads.
- Subjects :
- Marketing
Pharmacology
endocrine system
Organizational Behavior and Human Resource Management
Fetus
Germinal vesicle
Somatic cell
Strategy and Management
Pharmaceutical Science
Okadaic acid
Biology
Oogenesis
Molecular biology
In vitro
Andrology
chemistry.chemical_compound
Meiosis
chemistry
Epidermal growth factor
Drug Discovery
Subjects
Details
- ISSN :
- 18817211 and 1881607X
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Reproductive Immunology and Biology
- Accession number :
- edsair.doi...........a86d188f1651ebb6fd50e1c0405c3701