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Effect of dietary threonine on growth performance and muscle growth, protein synthesis and antioxidant-related signalling pathways of hybrid catfishPelteobagrus vachelli♀ ×Leiocassis longirostris♂
- Source :
- British Journal of Nutrition. 123:121-134
- Publication Year :
- 2019
- Publisher :
- Cambridge University Press (CUP), 2019.
-
Abstract
- The experiment was conducted to investigate the effects of dietary threonine (Thr) on growth performance and muscle growth, protein synthesis and antioxidant-related signalling pathways of hybrid catfishPelteobagrus vachelli♀ ×Leiocassis longirostris♂. A total of 1200 fish (14·19 (se0·13) g) were randomly distributed into six groups with four replicates each, fed six diets with graded level of Thr (9·5, 11·5, 13·5, 15·4, 17·4 and 19·3 g/kg diets) for 56 d. Results showed (P< 0·05) that dietary Thr (1) increased percentage weight gain, specific growth rate, feed efficiency and protein efficiency ratio; (2) up-regulated growth hormone (GH), insulin-like growth factor 1 (IGF-1), proliferating cell nuclear antigen, myogenic regulation factors (MyoD, Myf5, MyoG and Mrf4) and myosin heavy chain (MyHC) mRNA levels; (3) increased muscle protein content via regulating the protein kinase B/target of rapamycin signalling pathway and (4) decreased malondialdehyde and protein carbonyl contents, increased catalase, glutathione-S-transferase, glutathione reductase and GSH activities, up-regulated mRNA levels of antioxidant enzymes related to NFE2-related factor 2 andγ-glutamylcysteine ligase catalytic subunit. These results suggest that Thr has a potential role to improve muscle growth and protein synthesis, which might be due to the regulation of GH-IGF system, muscle growth-related gene, antioxidative capacity and protein synthesis-related signalling pathways. Based on the quadratic regression analysis of specific growth rate, the Thr requirement of hybrid catfish (14·19–25·77 g) was estimated to be 13·77 g/kg of the diet (33·40 g/kg of dietary protein).
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Nutrition and Dietetics
Protein efficiency ratio
Chemistry
Growth factor
medicine.medical_treatment
Glutathione reductase
Medicine (miscellaneous)
04 agricultural and veterinary sciences
MyoD
03 medical and health sciences
030104 developmental biology
Endocrinology
Internal medicine
Myosin
040102 fisheries
medicine
0401 agriculture, forestry, and fisheries
MYF5
Protein kinase B
Catfish
Subjects
Details
- ISSN :
- 14752662 and 00071145
- Volume :
- 123
- Database :
- OpenAIRE
- Journal :
- British Journal of Nutrition
- Accession number :
- edsair.doi...........a8355a8b7dfcc4bb62d08f91a95abae3