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Metabolomic analysis of intestinal epithelial cell maturation along the crypt–villus axis
- Source :
- RSC Advances. 6:27566-27574
- Publication Year :
- 2016
- Publisher :
- Royal Society of Chemistry (RSC), 2016.
-
Abstract
- Epithelial cells along the crypt–villus axis (CVA) undergo continual renewal through highly coordinated proliferation, differentiation, and apoptosis; however, the changes in metabolism during maturation along the CVA are still unclear. The present study investigates the global metabolite changes in intestinal epithelial cells during maturation along the CVA. Eight 21 day-old suckling piglets were used. Intestinal epithelial cells were isolated sequentially from the villus top to the bottom of the crypt with 6 fractions (F1 to F6), and GC-MS was used to identify the metabolites, whose levels changed. Three hundred metabolites were identified. PLS-DA and OPLS-DA analyses showed that the metabolism of intestinal epithelial cells gradually changed during maturation along the CVA. These analyses were also run to distinguish between two fractions of cells, and yielded good separation of F1 and F3, F1 and F4, F1 and F5, and F1 and F6 cells. Significant differences were found in the metabolism of fatty acids, amino acids, glucose, and other metabolites between villus cells and crypt cells. These results reveal a global change in cellular metabolism during maturation along the CVA, and provide basal information for understanding the mechanism involved for specific nutrients in regulating epithelial cell renewal and identifying nutrients to regulate mucosal morphology and functions.
- Subjects :
- 0301 basic medicine
chemistry.chemical_classification
General Chemical Engineering
Metabolite
Crypt
General Chemistry
Metabolism
Biology
Epithelium
Amino acid
Cell biology
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
medicine.anatomical_structure
Metabolomics
chemistry
Biochemistry
Apoptosis
medicine
Intestinal epithelial cell maturation
Subjects
Details
- ISSN :
- 20462069
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- RSC Advances
- Accession number :
- edsair.doi...........a6fb231b6ccf397e6a58a53747b4e79e