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3,5-Diethoxycarbonyl-1,4-Dihydrocollidine Diet: A Rodent Model in Cholestasis Research
- Source :
- Methods in Molecular Biology ISBN: 9781493994199
- Publication Year :
- 2019
- Publisher :
- Springer New York, 2019.
-
Abstract
- Liver cholestasis is characterized by impairment in bile flow. Among cholestatic diseases, primary biliary cholangitis and primary sclerosing cholangitis represent relevant causes of chronic liver disease, associated to significant morbidity and mortality. To better understand and to address therapeutic strategies to cholangiopathies is essential to develop an in vivo model which recapitulates the pathological features of the disease. Chronic feeding of 3,5-diethoxycarbonyl-1,4-dihydrocollidine, named DDC, has been proposed as an in vivo model for cholestatic disease due to the formation of intraductal porphyrin plugs. Chronic feeding of DDC in mice reproduces the main histopathological hallmarks of human cholestatic disease such as (1) remodeling of biliary compartments giving rise to ductular reaction, (2) periductular fibrosis, and (3) inflammatory infiltrate. This chapter describes the materials and methods necessary for the development and characterization of DDC diet-based mouse model.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
business.industry
Rodent model
Disease
medicine.disease
Chronic liver disease
Primary sclerosing cholangitis
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Cholestasis
In vivo
Fibrosis
medicine
030211 gastroenterology & hepatology
business
Pathological
Subjects
Details
- ISBN :
- 978-1-4939-9419-9
- ISBNs :
- 9781493994199
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781493994199
- Accession number :
- edsair.doi...........a67ae1180c06658adf4938ac4107e889