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A7.7 Different Genetic Background of Dermatomyositis and Polymyositis in a Single Centre Cohort
- Source :
- Annals of the Rheumatic Diseases. 72:A50.2-A50
- Publication Year :
- 2013
- Publisher :
- BMJ, 2013.
-
Abstract
- Background and Objectives The idiopathic inflammatory myopathies (IIM) are systemic connective tissue diseases in which autoimmune pathology is responsible for promotion of chronic muscle inflammation and weakness. As in many other autoimmune diseases, the development of IIM is also associated with genes of HLA complex. The aim of this study was to determine the basic relation between alleles of HLA genes and IIM. Materials and Methods We have performed low to high resolution genotyping to characterise the allelic profiles of HLA-DRB1, -DQB1 and -DQA1 loci in a large group of single centre cohort of patients suffering from IIM (n = 269). The genomic DNA was prepared by standard DNA extraction methods and the HLA typing was done using the commercial LABType® SSO kit (One Lambda, USA). Statistical evaluation of results was done with chi-2 test and Fisher exact test. Autoantibody profiles were analysed with radioactive immunoprecipitation. Results The frequencies of HLA-DRB1*03:01 and -DRB1*16:01 alleles were increased in IIM patients and the difference reached statistical significance when compared to healthy controls (P Presence of HLA-DRB1*03:01 allele was associated with anti-Jo-1, anti-Ro52, or anti-Pm-Scl positivity in all IIM patients (P The DRB1*16:01 allele was associated with negativity of all studied autoantibodies, particularly in subgroup of DM patients (P Conclusions This study identifies different genetic background between patients with dermatomyositis and polymyositis in a homogenous population of patients from a single centre. Acknowledgement This work is supported by Internal Grant Agency of Ministry of Health of the Czech Republic NT/12438–4 and NT/13699.
- Subjects :
- education.field_of_study
business.industry
Immunology
Population
Autoantibody
Human leukocyte antigen
Dermatomyositis
medicine.disease
Connective tissue disease
Polymyositis
General Biochemistry, Genetics and Molecular Biology
symbols.namesake
Rheumatology
symbols
medicine
Immunology and Allergy
education
business
Genotyping
Fisher's exact test
Subjects
Details
- ISSN :
- 14682060 and 00034967
- Volume :
- 72
- Database :
- OpenAIRE
- Journal :
- Annals of the Rheumatic Diseases
- Accession number :
- edsair.doi...........a634586935c9174c58b6eb022c44a466