Back to Search
Start Over
HPLC–UV analysis of sample preparation influence on flavonoid yield from Cirsium japonicum var. maackii
- Source :
- Applied Biological Chemistry. 60:519-525
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- This study was conducted to optimize the extraction conditions of flavonoids from Cirsium japonicum var. maackii (ICF-1). The effects of sample material ratio, solvent concentration, extraction time, solid-to-solvent ratio, and number of extractions on flavonoid extraction efficiency were analyzed. Three flavonoids were specifically investigated: cirsimarin (1), hispidulin (2), and cirsimaritin (3). In single-factor experiments, each variable had a significant effect on the determination of content of compounds 1–3. The optimal conditions for extraction were found to be: mass, 15 g; ratio of spring and fall leaves, 4:1; extraction solvent, 70% ethanol; extraction time, 4 h; solid-to-solvent ratio, 1:20; and number of extractions, 1. The results of the study were used to maximize the potential of ICF-1 samples and optimize the efficiency of the extraction process.
- Subjects :
- 0301 basic medicine
chemistry.chemical_classification
Chromatography
Chemistry
010401 analytical chemistry
Organic Chemistry
Flavonoid
Extraction (chemistry)
01 natural sciences
General Biochemistry, Genetics and Molecular Biology
0104 chemical sciences
Solvent
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Yield (chemistry)
Hispidulin
Sample preparation
Cirsium japonicum
Cirsimarin
Subjects
Details
- ISSN :
- 24680842 and 24680834
- Volume :
- 60
- Database :
- OpenAIRE
- Journal :
- Applied Biological Chemistry
- Accession number :
- edsair.doi...........a4bdc7c08d17fb2207bd02118c72a6f4