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Development of a multiplex real-time PCR method for early diagnosis of three bacterial diseases in fish: A real-case study in trout aquaculture
- Source :
- Aquaculture. 496:255-261
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Lactococcus garvieae, Yersinia ruckeri and Flavobacterium psychrophilum are three of the most important pathogens in worldwide rainbow trout aquaculture (Oncorrhynchus mykiss Walbaum). In this work, a multiplex quantitative PCR (qPCR) method for the simultaneous detection of the three pathogens was developed and tested in a commercial trout farm. Fifty-four spleen and brain samples from a trout farm were analyzed using both multiplex qPCR and classic microbiological methods. When comparing qPCR results and classic plate diagnostic techniques no negative deviations were observed, which resulted in a 100% relative sensitivity of the multiplex qPCR method developed. In addition, efficiency of the qPCR method ranged between 97.5% and 108.8%. The specificity of the combination of primers and probes was successfully tested in 57 target and non-target bacterial strains Hence, the multiplex qPCR method developed in the present study could be used as a reliable diagnostic tool for the detection of L. garvieae, Y. ruckeri and F. psychrophilum in rainbow trout farms, enabling faster diagnostics (only a few hours) and contributing to a quick response and management of bacterial disease outbreaks.
- Subjects :
- 0301 basic medicine
Bacterial disease
biology
business.industry
animal diseases
030106 microbiology
Flavobacterium psychrophilum
Aquatic Science
biology.organism_classification
Microbiology
03 medical and health sciences
Trout
Aquaculture
Lactococcus garvieae
Multiplex
Rainbow trout
Yersinia ruckeri
business
Subjects
Details
- ISSN :
- 00448486
- Volume :
- 496
- Database :
- OpenAIRE
- Journal :
- Aquaculture
- Accession number :
- edsair.doi...........a47e79915acf709db24664c328987e90