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Double‐stranded <scp>RNAs</scp> targeting HvRPS18 and HvRPL13 reveal potential targets for pest management of the 28‐spotted ladybeetle, Henosepilachna vigintioctopunctata
- Source :
- Pest Management Science. 76:2663-2673
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Background RNA interference (RNAi) is a potential tool for plant protection against insect pests. The great challenge for effective pest control using RNAi in the field is the development of efficient and reliable methods for the production and delivery of double-stranded RNA (dsRNA). Results In the present study, we investigated the potential of feeding in vitro synthesized or bacterially expressed dsRNA to populations of the 28-spotted ladybeetle Henosepilachna vigintioctopunctata as a method of biological pest control. Ingestion of in vitro synthesized dsHvRPS18 or dsHvRPL13 led to significant down-regulation of the ribosomal protein-encoding genes HvRPS18 and HvRPL13, respectively, and significantly decreased the survival of H. vigintioctopunctata. Such silencing of HvRPS18 or HvRPL13 expression appeared to be partially dose-dependent and also inhibited the growth of H. vigintioctopunctata and significantly suppressed the expression of digestive enzyme-related genes. Finally, ingestion of bacterially expressed dsHvRPS18 or dsHvRPL13 induced significant mortality in the first and third instars, and in adults. Conclusion The effectiveness of RNAi-based gene silencing in H. vigintioctopunctata provides a powerful reverse genetic tool for the functional annotation of its genes. This study demonstrates that HvRPS18 and HvRPL13 represent candidate genes for RNAi-based biological control of H. vigintioctopunctata. © 2020 Society of Chemical Industry.
- Subjects :
- 0106 biological sciences
biology
business.industry
fungi
Henosepilachna vigintioctopunctata
Pest control
RNA
General Medicine
Ribosomal RNA
biology.organism_classification
01 natural sciences
Cell biology
010602 entomology
RNA silencing
RNA interference
Insect Science
Gene silencing
business
Agronomy and Crop Science
Gene
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15264998 and 1526498X
- Volume :
- 76
- Database :
- OpenAIRE
- Journal :
- Pest Management Science
- Accession number :
- edsair.doi...........a37b6a1dc3f3f05b38af1ce98252e7d2