Selective medium for the isolation of Erwinia ananas, the causal agent of bacterial pales browning of rice

A selective medium (designated as NSVC-In medium) was developed to isolate of Erwinia ananas, the causal agent of bacterial palea browning of rice. Medium NSVC-In contains per 1000ml of distilled water: 10g inositol, 10g peptone, 50g NaCl, 100mg vancomycin, 100mg cycloheximide, and 15g agar. The medium is adjusted to pH 6.8 with NaOH before autoclaving. Plant pathogenic E. ananas grew well on this medium to develop characteristic colonies, but the growth of nonpathogenic E. ananas and Erwinia herbicola was inhibited. Plating efficiency of this medium for pathogenic E. ananas was the same as that of nutrient agar medium. Pathogenic E. ananas could be selectively detected from dead leaves of rice plants.