Aerobic Bacterial Isolates and Their Antibiotic Susceptibility Patterns of Pus Samples at Tertiary Healthcare Center of Northern India

Introduction: Emerging resistance in microorganisms is a matter of great concern in health care setups. Bacterial isolate and AMR pattern changes from place to place and time to time. Unprofessional use of antibiotics has resulted in an increasing burden of antibiotic resistance. This study focused over the aerobic bacteriological profile and antibiotic resistance pattern in pus isolates. Materials and Methods: A retrospective study was carried out from June, 2020 to May 2021. 1174 pus samples collected during the study period were included. The samples were cultured on Blood and MacConkey agar. After aerobic incubation at 37°C for 18-24 hrs, organisms were identified by biochemical reactions, gram staining and antibiotic susceptibility was tested by Kirby Bauer disc diffusion method. Result: Among the total 1174 pus samples, 712 (60.6%) were male and 462 (39.3%) were females. 857(72.9%) samples showed positive results. Among them, Staphylococcus aureus was the most common isolate followed by Escherichia coli, Pseudomonas spp. and Klebsiella spp. The sensitivity of Staphylococcus aureus was highest with Vancomycin and Linezolid, whereas Enterobacterales and Pseudomonas were sensitive with Piperacillin tazobactam. Conclusion: Increasing resistance always poses a challenge in treating infections. The development of resistant strains of pathogens can be limited by the judicious use of antibiotics. This study shows the infecting bacterial isolates and their antibiogram from this area. Keywords: Pus, antimicrobial resistance, bacterial isolates.