Increased Anticancer Activity by the Surfated Funcoidan from Korean Brown Seaweeds

Department of Biology, Pusan National University, Pusan 609-735, Korea Received January 31, 2002) . Fucoidan ! , "#$ ! , "% ! , &' ! , virus ! ( !,- )*+ $ ./ 01 2 3. /$ 4 . 5 67 fucoidan- 89 :; !:; CD ?@AB > *+ fucoidanE G F. HIJ - KL :$ - M @NO ? . C P FGE - .QR ' fucoidan> FGE - R STQ fucoidanA *+> V$ U , > RPAE ssDNA W ,- X G Y) >[ Z7 \ ] ^ . 5 3 J_ 89J G= F J fucoidanE - `, *+ O A;a . : F fucoidan, b , replication protein A(RPA), SV40 DNA cSABSTRACT.Fucoidan is a kind of polysaccharides in brown seaweeds. For the past years have beenextensively studied due to their numerous biological activities : anticancer, anticoagulant, antithrombotic, anti-inflammatory and antiviral. In this study, we have extracted fucoidan from the Korean brown seaweeds andexamined it’s anticancer activities for employed SV40 DNA replication assay, RPA-ssDNA binding assay ofreplication protein A(RPA: known as human single-stranded DNA-binding protein essential for DNA rep-lication) and MCF7 cell growth inhibition assay. In addition to, we found that chemically sulfated fucoidan'santicancer activity is more higher than natural and desulfated fucoidan. It seem that fucoidan's sulfate groupaffect on DNA replication, cause of decrease RPA's DNA binding activity. These results suggests that sulfatedfucoidan from Korean brown seaweeds have anticancer activity.Key words: Sulfated fucoidan, anticancer, replication protein A(RPA), SV40 DNA replication