Estimation of Azadirachtin Content in Neem Extracts and Formulations

A high performance liquid chromatographic reversed-phase procedure has been developed whereby azadirachtin content can be estimated in crude extracts of neem and in dust formulations of neem. An estimation of the azadirachtin content is achieved through the use of an external azadirachtin standard and valley-to-valley integration. Since azadirachtin seems to be the most potent insect feeding deterrent in these extracts and formulations, its content is a measurement of potency and represents an attempt at standardization.