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Capsid-like particles decorated with the SARS2-CoV-2 receptor-binding domain elicit strong virus neutralization activity

Authors :
Cyrielle Fougeoux
Louise Goksøyr
Manja Idorn
Vladislav Soroka
Sebenzile K. Myeni
Robert Dagil
Christoph M. Janitzek
Teit Søgaard
Kara-Lee Aves
Emma W. Horsted
Sayit Mahmut Erdoğan
Tobias Gustavsson
Jerzy Dorosz
Stine Clemmensen
Laurits Larsen
Susan Thrane
Elena E. Vidal-Calvo
Paul Khalifé
Thomas M. Hulen
Swati Choudhary
Michael Theisen
Susheel Singh
Asier Garcia-Senosiain
Linda Van Oosten
Gorben Pijlman
Bettina Hierzberger
Tanja Domeyer
Blanka W. Nalewajek
Anette Strøbæk
Magdalena Skrzypczak
Laura F. Andersson
Tim Dalebout
Kasper Iversen
Lene H. Harritshøj
Benjamin Mordmüller
Henrik Ullum
Line Reinert
Willem Adriaan de Jongh
Marjolein Kikkert
Soren Paludan
Thor Theander
Morten Nielsen
Ali Salanti
Adam Sander
Publication Year :
2020
Publisher :
Research Square Platform LLC, 2020.

Abstract

The rapid development of a SARS-CoV-2 vaccine is a global priority. Here, we developed two capsid-like particle (CLP)-based vaccines displaying the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. RBD antigens were displayed on AP205 CLPs through a novel split-protein Tag/Catcher ensuring unidirectional and high-density display of RBD. Both soluble recombinant RBD, and RBD displayed on CLPs bound the ACE2 receptor with nanomolar affinity. Mice were vaccinated with soluble RBD or CLP-displayed RBD, formulated in Squalene-Water-Emulsion. The RBD-CLP vaccines induced higher levels of serum anti-RBD antibodies, than the soluble RBD vaccines. Remarkably, one injection with our lead RBD-CLP vaccine in mice elicited virus neutralization antibody titers comparable to those found in patients which had recovered from Covid-19. Following booster vaccinations, the virus neutralization titers exceeded those measured after natural infection, at serum dilutions above 1:10.000. Thus, the RBD-CLP vaccine is highly promising candidates for preventing COVID-19 disease.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........9fa692aa0e0459551e3a1c2cc1dc4e00
Full Text :
https://doi.org/10.21203/rs.3.rs-45062/v1