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Advantages and Possibilities of Fluorescence-Based Methods for the Visualization of Apoptosis and Autophagy in Human Tumor Cells in vitro
- Source :
- Optics and Spectroscopy. 126:693-702
- Publication Year :
- 2019
- Publisher :
- Pleiades Publishing Ltd, 2019.
-
Abstract
- The possibilities of using fluorescence-based analytical methods and their advantages for visualization and identification of the type of programmed cell death in human tumor cells exposed to flavonoids have been analyzed in experiments in vitro. HeLa cervical cancer cells and A498 kidney carcinoma cells were used as the objects of the study, and exposure to the flavonoid-containing extract of common hedgehyssop (Gratiola officinalis L.) was tested as the experimental treatment. The following fluorescence-based techniques were used: the “live and dead” test with double staining by propidium iodide and acridine orange and double staining with annexin and propidium iodide. Autophagy induction was confirmed by fluorescence-based tests implemented in a Muse cell analyzer with a Muse Autophagy LC3-Antibody Based Kit. The use of double staining with acridine orange and propidium iodide fluorescent dyes in the “live and dead” test and comparison with phase contrast microscopy enables the visualization of apoptotic body and autophagosome formation processes in the cells and can, therefore, be used as a method of screening assessment of the efficiency of various chemotherapy drugs.
- Subjects :
- 010302 applied physics
Programmed cell death
Muse cell
biology
Autophagy
Acridine orange
biology.organism_classification
Apoptotic body
01 natural sciences
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Cell biology
010309 optics
HeLa
chemistry.chemical_compound
chemistry
Apoptosis
0103 physical sciences
Propidium iodide
Subjects
Details
- ISSN :
- 15626911 and 0030400X
- Volume :
- 126
- Database :
- OpenAIRE
- Journal :
- Optics and Spectroscopy
- Accession number :
- edsair.doi...........9f7c12785ddf327e1e457cc6823d503b