Nanosecond-Duration Electric Pulses Open Nanometer-Size Pores in Cell Plasma Membrane

We found that ultra-short electric pulses (60 or 600 ns duration) applied to mammalian cells cause profound, dose-dependent increase of plasma membrane electrical conductance. This effect is detectable even minutes after the exposure and is explained by formation of long-lived, voltagesensitive, inward-rectifying pores of nanometer diameter (”nanopores”). The phenomenon of nanoelectroporation and the extended lifetime of nanopores can also be demonstrated by a surge of Tl + uptake in the presence of K + channel blockers, as well as in CHO cells that express no endogenous voltage-gated K + channels. Due to their long lifetime, nanopores can have significant impact on cell physiology.