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Investigation of biotransformation of selenium in plants using spectrometric methods
- Source :
- Spectrochimica Acta Part B: Atomic Spectroscopy. 130:7-16
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- The aim of this research was to study the processes of biotransformation of selenium in plants such as garlic, radish sprouts and sunflower sprouts via identification of selenium-containing compounds as metabolites of inorganic selenium using mass spectrometry. Speciation analysis of selenium in extracts from plant samples was performed with the use of hyphenated high performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC-ICP-MS) method. Matching the retention times of sample compounds with standards allowed identification of Se-methyl-selenocysteine, selenomethionine, γ-glutamyl-Se-methylselenocysteine and inorganic SeO32 −. However, registered chromatograms included additional 82Se signals which couldn't be identified due to the lack of standards. Qualitative analysis of unknown compounds was achieved using high-resolution mass spectrometer equipped with mass analyzer Orbitrap coupled to high performance liquid chromatography. Since selenium has six stable isotopes of different abundance in nature, mass spectra of have a very characteristic isotopic pattern. In order to elucidate the structure of unknown Se compounds, selected ions were subjected to the fragmentation. Following selenocompounds were identified an inorganic selenium metabolites in garlic, sunflower sprouts and/or radish sprouts: selenohomolanthionine, Se-methyl-selenocysteine, selenomethionine, selenomethionine oxide, deaminohydroxy-selenohomolanthionine, N-acetylcysteine-selenomethionine, γ-glutamyl-Se-methyl-selenocysteine, methylseleno-Se-pentose-hexose, Se-methyl-selenoglutathione, 2,3-dihydroxy-propionyl-selenocysteine-cysteine, methyltio-selenoglutathione, 2,3-dihydroxypropionyl-selenolanthionine and two Se-containing compounds with proposed molecular formula C10H18N2O6Se and C10H13N5O3Se. Moreover, the structure was proposed for one selenocompound found in sunflower sprouts which has not been reported so far.
- Subjects :
- Chromatography
010401 analytical chemistry
food and beverages
chemistry.chemical_element
010402 general chemistry
Orbitrap
Mass spectrometry
01 natural sciences
High-performance liquid chromatography
Sunflower
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Analytical Chemistry
law.invention
chemistry
Biotransformation
law
Mass spectrum
Instrumentation
Inductively coupled plasma mass spectrometry
Spectroscopy
Selenium
Subjects
Details
- ISSN :
- 05848547
- Volume :
- 130
- Database :
- OpenAIRE
- Journal :
- Spectrochimica Acta Part B: Atomic Spectroscopy
- Accession number :
- edsair.doi...........9ce0bcf4860fb7be60c231a0fe32e9ef