H4K20me3 and H3K79me3 epigenetic changes in Endometriosis and endometrial tissues (LB844)

The significance of epigenetic changes in the etiology and pathogenesis of endometriosis is being progressively recognized. However, the function of lysine-histone epigenetic modifications and furthermore, their role in the pathology of this disease remains unclear. We lead a study aimed to identify specific epigenetic profiles and evaluate possible associations with gene expression to provide insights about the pathological processes occurring in endometriosis. Objective: Identify the methylation pattern of lysine-histones H4K20me3 and H3K79me3 and chromatin modulation by gene activation in endometrial tissues. Methods: We used endometrial tissues from 9 women, with and without endometriosis. Macro-dissection was achieved by following pathology protocols. Tissues were homogenized and disaggregated using a bullet blender. Total histone extraction was carried out using an Epiquick Kit (Epigentek) and then, western blots (WB) were performed. Both, the H4K20me3 and H3K79me3 were detected in all endometrial t...