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Unilateral ureteral obstruction evokes renal tubular apoptosis via the enhanced oxidative stress and endoplasmic reticulum stress in the rat
- Source :
- Neurourology and Urodynamics. 30:472-479
- Publication Year :
- 2011
- Publisher :
- Wiley, 2011.
-
Abstract
- Purpose Oxidative stress and endoplasmic reticulum (ER) stress may induce renal apoptosis and contribute to the pathogenesis of the kidney with unilateral ureteral obstruction (UUO). Materials and Methods We induced UUO the female Wistar rats by ligation of the left ureter at the ureteropelvic junction. The UUO kidney was performed from 4 hr to 7 days course. At the indicated time, we measured the arterial blood pressure and renal blood flow in each rat, renal ROS measurement in vivo by a chemiluminescence analyzer. We performed immunohistochemistry of monocyte/macrophage (ED-1) stain for leukocyte infiltration, 4-hydroxynoneal (4-HNE) stain for ROS products, and apoptosis by terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) and Western blot to analyze ER stress-associated and apoptosis-related proteins expression in the UUO kidney. Results We found that UUO decreased renal blood flow and increased renal vascular resistance and renal ROS. UUO decreased renal manganese superoxide dismutase (MnSOD) and catalase protein expression in a time-dependent manner. Increased 4-HNE stain in the renal tubules and ED-1 stain in the renal tubulointerstitial compartment occurred after 4 hr of UUO in the kidney. UUO significantly enhanced ER stress markers like ER stress-response protein 25 and glucose-regulated protein 78 and ER-associated apoptosis proteins, c-JUN NH2-terminal kinase, and caspase 12, in the kidney. Subsequently, UUO enhanced renal pro-apoptotic Bax and caspase 3 expression and decreased anti-apoptotic Bcl-2 expression, leading to renal tubular apoptosis. Conclusions Our data suggest that renal tubular apoptosis induced by oxidative stress and ER stress occurred in the UUO kidney. Neurourol. Urodynam. 30:472–479, 2011. © 2011 Wiley-Liss, Inc.
- Subjects :
- medicine.medical_specialty
Kidney
TUNEL assay
Renal circulation
biology
urogenital system
business.industry
Urology
Caspase 3
urologic and male genital diseases
medicine.disease_cause
female genital diseases and pregnancy complications
Endocrinology
medicine.anatomical_structure
Internal medicine
Renal blood flow
biology.protein
medicine
Unfolded protein response
Neurology (clinical)
business
Caspase 12
Oxidative stress
Subjects
Details
- ISSN :
- 07332467
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Neurourology and Urodynamics
- Accession number :
- edsair.doi...........9b633e6476f52f1acbd67d0d2393e3d4
- Full Text :
- https://doi.org/10.1002/nau.20855