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MicroRNA expression in hepatocellular carcinoma after the eradication of chronic hepatitis virus C infection using interferon therapy
- Source :
- Hepatology Research. 46:E26-E35
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- Aim Hepatocellular carcinoma (HCC) develops in up to 5% of patients after the successful treatment of chronic hepatitis C virus (HCV) infection using interferon therapy. The aim of this study was to characterize miRNA expression in liver tissues from patients who achieved a sustained viral response (SVR). Methods Seventy-one patients with resected HCC were enrolled into the present study: 61 HCC from patients with continuously infected HCV (HCV-HCC) and 10 from patients who had achieved SVR (SVR-HCC). We also included non-tumor tissues (SVR-NT) from four patients with SVR-HCC, and liver tissue (SVR-CH) from four SVR patients without HCC. Total RNA was extracted from liver samples. The miRNA expression patterns were analyzed using microarrays. In addition, target gene expression was quantified after miRNA overexpression in HEK293 cells. Results We could discriminate between SVR-HCC and HCV-HCC with 75.36% accuracy using the expression pattern of six specific miRNA. The expression levels of 37 miRNA were significantly lower in HCV-HCC than in SVR-HCC, whereas the expression of 25 miRNA was significantly higher in HCV-HCC than SVR-HCC (P
- Subjects :
- 0301 basic medicine
Hepatology
business.industry
Hepatitis C virus
HEK 293 cells
virus diseases
medicine.disease
medicine.disease_cause
digestive system diseases
Virus
03 medical and health sciences
030104 developmental biology
Infectious Diseases
Interferon
Hepatocellular carcinoma
Thrombospondin 1
microRNA
Immunology
Cancer research
medicine
DNA microarray
business
neoplasms
medicine.drug
Subjects
Details
- ISSN :
- 13866346
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- Hepatology Research
- Accession number :
- edsair.doi...........9a87f31bd83f855bd9343a901582bdde