DIFFERENT POLYPHENOLS HAVE DIFFERENT AFFINITIES FOR C‐TERMINAL SUBUNITS OF MALTASE‐GLUCOAMYLASE AND SUCRASEISOMALTASE FOR THE MODULATION OF GLUCOSE RELEASE

Some polyphenolic compounds inhibit the intestinal alpha-glucosidases. However, it is not clear how those compounds affect the enzyme activity of the individual C or N terminals subunits of maltase-glucoamylase (Mgam) and sucrase-isomaltase (Si). ObjectiveTo modulate the enzymatic activity of the most active C terminal-Mgam (Ct-Mgam) and C terminal Si (Ct-Si) by selected polyphenols in order to control the glucose release from the starch digestion. MethodsMaltase activities of recombinant Ct-Mgam and Ct-Si subunits were measured by Tris Glucose- Oxidase (TGO) method. The reactions were stopped by TGO and the optical density was measured at 450 nm. ResultsMaltase activity of both enzymes was inhibited. IC50 values were 0.58 and 0.571 mM for caffeic acid, 0.039 and 0.014 mM chlorogenic acid, 0.139 and 0.213 mM for (+)-catechin hydrate, 0.383 and 0.177 mM for gallic acid, 0.041 and 0.014 mM for (−) epigallocatechin gallate for Ct-Si and Ct-Mgam, respectively. ConclusionsThe results indicate that the potencie...