Back to Search
Start Over
An efficient method for DNA isolation from red algae
- Source :
- Journal of Applied Phycology. 16:161-166
- Publication Year :
- 2004
- Publisher :
- Springer Science and Business Media LLC, 2004.
-
Abstract
- A simple, inexpensive and efficient method was developed for rapid isolation of total genomic DNA from 15 red algal species. It resulted in 0.1 mug high quality DNA from 1 mg fresh algal material, with an A(260)/A(280) ratio of 1.68 - 1.90. Using this rapidly isolated DNA, the 18S ribosomal RNA genes ( rDNA) and the nuclear ribosomal DNA of the internal transcribed spacer (ITS) regions were amplified. The tested DNA was suitable for restriction endonuclease digestion, genetic marker analysis and polymerase chain reaction (PCR) amplification, and may be valid for other genetic manipulation.
Details
- ISSN :
- 09218971
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Journal of Applied Phycology
- Accession number :
- edsair.doi...........99033e97c84bbd66cf7d0223590cc490