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Multicolor biosensor for fish freshness assessment with the naked eye

Authors :
Xiaoming Ma
Yue Lin
Guonan Chen
Zhitao Chen
Bin Qiu
Longhua Guo
Zhenyu Lin
Source :
Sensors and Actuators B: Chemical. 252:201-208
Publication Year :
2017
Publisher :
Elsevier BV, 2017.

Abstract

Fish product is one of the main seafood with the disadvantage of easy to perish. Various indexes and diverse methods have been developed to evaluate the fish freshness, but most of them are complex and cannot be applied for on-field detection. In this study, we reported a simple, visual and economical multicolor biosensor based on the etching of gold nanorods (GNRs) to evaluate the fish freshness with the naked eye. Hypoxanthine (Hx) is chosen as the index of fish freshness and it react with the dissolved oxygen to produce H 2 O 2 at the presence of xanthine oxidase (XOD). Then GNRs are etched quickly by the H 2 O 2 in the presence of Fe 2+ . Correspondingly, the GNRs surface plasmon resonance (SPR) is regulated and results in a distinctly color change of the system, which can be easily distinguished with the naked eye. Therefore, the concentration of Hx in the fish samples can be semi-quantitatively analyzed with the naked eye. The proposed multicolor Hx sensor has been successfully applied to the detection of Hx in fish samples.

Details

ISSN :
09254005
Volume :
252
Database :
OpenAIRE
Journal :
Sensors and Actuators B: Chemical
Accession number :
edsair.doi...........98998f5d6222b2582182c15737a4b8ea
Full Text :
https://doi.org/10.1016/j.snb.2017.06.007