Back to Search
Start Over
Dual HER2 Gene Protein Assay
- Source :
- American Journal of Clinical Pathology. 143:451-458
- Publication Year :
- 2015
- Publisher :
- Oxford University Press (OUP), 2015.
-
Abstract
- Objectives: The combined gene protein assay (GPA) can simultaneously assess HER2 gene copy number and protein on a single slide using bright-field microscopy. Methods: GPA was compared with a fluorescence in situ hybridization (FISH) assay on 50 invasive breast carcinomas with a 2+ score on immunohistochemistry (IHC). Results: The cases were categorized into positive, equivocal, or negative for HER2 gene amplification using the 2013 American Society of Clinical Oncology/College of American Pathologists criteria. This resulted in 82% agreement (41 of 50) between FISH and GPA. In addition, 25 known IHC 3+ breast carcinomas analyzed by GPA showed protein overexpression and clusters of HER2 gene consistent with unequivocal amplification, and 22 known IHC-negative cases were negative for HER2 gene amplification by GPA. Conclusions: Although GPA is an alternative to both IHC and FISH, it may be an unnecessary test for IHC 0/1+/3+ cases. The clinical utility of GPA appears to be similar to other in situ hybridization assays (ie, adjudicator of HER2 status for IHC 2+ cases).
- Subjects :
- medicine.diagnostic_test
macromolecular substances
General Medicine
In situ hybridization
Biology
medicine.disease
Molecular biology
Breast cancer
stomatognathic system
medicine
Fish
Immunohistochemistry
Copy-number variation
skin and connective tissue diseases
Gene
Bradford protein assay
Fluorescence in situ hybridization
Subjects
Details
- ISSN :
- 19437722 and 00029173
- Volume :
- 143
- Database :
- OpenAIRE
- Journal :
- American Journal of Clinical Pathology
- Accession number :
- edsair.doi...........97e53dbc291f4255c27db6bd8e2ed818