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Molecular typing of drug-susceptible and -resistant Mycobacterium tuberculosis in Johannesburg, South Africa
- Source :
- The International Journal of Tuberculosis and Lung Disease. 19:834-840
- Publication Year :
- 2015
- Publisher :
- International Union Against Tuberculosis and Lung Disease, 2015.
-
Abstract
- SETTING Knowledge about spoligotyping families of drug-susceptible and drug-resistant Mycobacterium tuberculosis circulating in the Johannesburg area, South Africa, is lacking. OBJECTIVE To determine the genetic diversity of M. tuberculosis isolates circulating in the Johannesburg area and to compare the results with both national and international databanks. DESIGN Five hundred cultured M. tuberculosis isolates from within the greater Johannesburg metropolitan area collected from January 2009 to December 2010 were obtained from the National Health Laboratory Services (NHLS) Mycobacteriology Referral Laboratory, Johannesburg, in MGIT vials. The isolates were specimens from individuals with tuberculosis (TB) symptoms and known TB patients submitted to the NHLS for routine mycobacterial culture and drug susceptibility testing. The isolates were genotyped using spoligotyping. RESULTS Spoligotyping generated 62 shared types, with 92% (458/500) of the sample size matching pre-existing shared types. Of the 62 shared types, eight families were predominant (clustering from 16 to 132), representing 64% (340/500) of the sample. The Beijing family (135/500) predominated (P < 0.001). CONCLUSION TB incidence in Johannesburg does not appear to be due to clonality, but is rather due to diverse circulating strains, namely the Beijing family, followed by the S, Latin American-Mediterranean and T families.
- Subjects :
- Pulmonary and Respiratory Medicine
National health
medicine.medical_specialty
Tuberculosis
biology
business.industry
Incidence (epidemiology)
Mycobacterial culture
Drug susceptibility
medicine.disease
biology.organism_classification
Mycobacterium tuberculosis
Beijing family
Molecular typing
Infectious Diseases
Internal medicine
medicine
business
Subjects
Details
- ISSN :
- 18157920 and 10273719
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- The International Journal of Tuberculosis and Lung Disease
- Accession number :
- edsair.doi...........97b48dae5a671d3ffcb5ef5ce7e7980c