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Chemotaxonomy of Trichoderma spp. Using Mass Spectrometry-Based
- Source :
- Journal of Microbiology and Biotechnology. 21:5-13
- Publication Year :
- 2011
- Publisher :
- Journal of Microbiology and Biotechnology, 2011.
-
Abstract
- In this study, seven Trichoderma species (33 strains) were classified using secondary metabolite profile-based chemotaxonomy. Secondary metabolites were analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS-MS) and multivariate statistical methods. T. longibrachiatum and T. virens were independently clustered based on both internal transcribed spacer (ITS) sequence and secondary metabolite analyses. T. harzianum formed three subclusters in the ITS-based phylogenetic tree and two subclusters in the metabolitebased dendrogram. In contrast, T. koningii and T. atroviride strains were mixed in one cluster in the phylogenetic tree, whereas T. koningii was grouped in a different subcluster from T. atroviride and T. hamatum in the chemotaxonomic tree. Partial least-squares discriminant analysis (PLS-DA) was applied to determine which metabolites were responsible for the clustering patterns observed for the different Trichoderma strains. The metabolites were hetelidic acid, sorbicillinol, trichodermanone C, giocladic acid, bisorbicillinol, and three unidentified compounds in the comparison of T. virens and T. longibrachiatum; harzianic acid, demethylharzianic acid, homoharzianic acid, and three unidentified compounds in T. harzianum I and II; and koninginin B, E, and D, and six unidentified compounds in T. koningii and T. atroviride. The results of this study demonstrate that secondary metabolite profiling-based chemotaxonomy has distinct advantages relative to ITSbased classification, since it identified new Trichoderma clusters that were not found using the latter approach.
- Subjects :
- Phylogenetic tree
biology
Metabolite
Dendrogram
Fungal genetics
General Medicine
Secondary metabolite
biology.organism_classification
Applied Microbiology and Biotechnology
chemistry.chemical_compound
chemistry
Chemotaxonomy
Trichoderma
Botany
medicine
Internal transcribed spacer
Biotechnology
medicine.drug
Subjects
Details
- ISSN :
- 17388872 and 10177825
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Journal of Microbiology and Biotechnology
- Accession number :
- edsair.doi...........96e805f231690b3260b66a88b2c33c28