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Validation of PM2.5 model particle through physicochemical evaluation and atherosclerotic plaque formation in ApoE-/- mice
- Source :
- Ecotoxicology and Environmental Safety. 192:110308
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- PM2.5 particles are regarded as prominent risk factors that contribute to the development of atherosclerosis. However, the composition of PM2.5 is rather complicated. This study aimed to provide a model particle that simulates the behavior of actual PM2.5, for subsequent use in exploring mechanisms and major complications arising from PM2.5. To establish model particles of PM2.5, a series of monodisperse SiO2 microspheres with different average grain diameters were mixed according to the size distribution of actual PM2.5. The organic carbon (OC) was removed from PM2.5 and coated onto the SiO2 model particle, to formulate simulant PM2.5. Results showed that the size distribution of the model particle was highly approximate to that of the PM2.5 core. The polycyclic aromatic hydrocarbon (PAHs) composition profile of the simulated PM2.5 were approximate to PM2.5, and loading efficiency was approximately 80%-120%. Furthermore, compared to the control, SiO2-only model particle had negligible cytotoxicity on cell viability and oxidative stress of HUVECs, and marginal effect on the lipid metabolism and atherosclerotic plaque formation in ApoE-/- mice. In contrast, simulated PM2.5 exhibited similar cytotoxic and detrimental effects on lipid metabolism and atherosclerotic plaque formation with actual PM2.5. Traffic-related PM2.5 had negative effects on endothelial function and led to the formation of atherosclerosis via oxidative stress. The simulated PM2.5 simulated the outcomes of actual PM2.5 exposure. Here, we show that SiO2 particle model cores coated with OC could significantly assist in the evaluation of the effects of specific organic compositions bound on PM2.5, specifically in the context of environmental health and safety.
- Subjects :
- chemistry.chemical_classification
021110 strategic, defence & security studies
Apoe mice
Health, Toxicology and Mutagenesis
Dispersity
0211 other engineering and technologies
Public Health, Environmental and Occupational Health
Polycyclic aromatic hydrocarbon
Context (language use)
Lipid metabolism
02 engineering and technology
General Medicine
010501 environmental sciences
medicine.disease_cause
complex mixtures
01 natural sciences
Pollution
chemistry
Biophysics
medicine
Particle
Viability assay
Oxidative stress
0105 earth and related environmental sciences
Subjects
Details
- ISSN :
- 01476513
- Volume :
- 192
- Database :
- OpenAIRE
- Journal :
- Ecotoxicology and Environmental Safety
- Accession number :
- edsair.doi...........959a5fb5d8dfbf12561a6812df6848ce