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Targeting the epitope spreader Pep19 by naïve human CD45RA+ regulatory T cells dictates a distinct suppressive T cell fate in a novel form of immunotherapy
- Source :
- Journal of Periodontal & Implant Science. 47:292
- Publication Year :
- 2017
- Publisher :
- Korean Academy of Periodontology, 2017.
-
Abstract
- Purpose Beyond the limited scope of non-specific polyclonal regulatory T cell (Treg)-based immunotherapy, which depends largely on serendipity, the present study explored a target Treg subset appropriate for the delivery of a novel epitope spreader Pep19 antigen as part of a sophisticated form of immunotherapy with defined antigen specificity that induces immune tolerance. Methods Human polyclonal CD4+CD25+CD127lo- Tregs (127-Tregs) and naive CD4+CD25+CD45RA+ Tregs (45RA-Tregs) were isolated and were stimulated with target peptide 19 (Pep19)-pulsed dendritic cells in a tolerogenic milieu followed by ex vivo expansion. Low-dose interleukin-2 (IL-2) and rapamycin were added to selectively exclude the outgrowth of contaminating effector T cells (Teffs). The following parameters were investigated in the expanded antigen-specific Tregs: the distinct expression of the immunosuppressive Treg marker Foxp3, epigenetic stability (demethylation in the Treg-specific demethylated region), the suppression of Teffs, expression of the homing receptors CD62L/CCR7, and CD95L-mediated apoptosis. The expanded Tregs were adoptively transferred into an NOD/scid/IL-2Rγ-/- mouse model of collagen-induced arthritis. Results Epitope-spreader Pep19 targeting by 45RA-Tregs led to an outstanding in vitro suppressive T cell fate characterized by robust ex vivo expansion, the salient expression of Foxp3, high epigenetic stability, enhanced T cell suppression, modest expression of CD62L/CCR7, and higher resistance to CD95L-mediated apoptosis. After adoptive transfer, the distinct fate of these T cells demonstrated a potent in vivo immunotherapeutic capability, as indicated by the complete elimination of footpad swelling, prolonged survival, minimal histopathological changes, and preferential localization of CD4+CD25+ Tregs at the articular joints in a mechanistic and orchestrated way. Conclusions We propose human naive CD4+CD25+CD45RA+ Tregs and the epitope spreader Pep19 as cellular and molecular targets for a novel antigen-specific Treg-based vaccination against collagen-induced arthritis.
- Subjects :
- 0301 basic medicine
Adoptive cell transfer
Regulatory T cell
medicine.medical_treatment
T cell
FOXP3
hemic and immune systems
chemical and pharmacologic phenomena
030206 dentistry
Immunotherapy
Biology
Epitope
Immune tolerance
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
medicine.anatomical_structure
Immunology
Cancer research
medicine
Periodontics
IL-2 receptor
Oral Surgery
Subjects
Details
- ISSN :
- 20932286 and 20932278
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- Journal of Periodontal & Implant Science
- Accession number :
- edsair.doi...........956bf444dec825b00247796106ce1b53
- Full Text :
- https://doi.org/10.5051/jpis.2017.47.5.292