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The measurement of hydrophobic polypeptides in beer using the fluorochrome 1-anilino-8-naphthalenesulfonate
- Source :
- Food Chemistry. 75:377-383
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- A method has been explored for assessing hydrophobic polypeptides in beer by measuring fluorescence developed upon interaction of the proteins with 1-anilino-8-naphthalenesulfonate. The method was initially applied to three separate purified proteins (bovine serum albumin, cytochrome c and catalase) and different response patterns were observed, suggesting that a simple picture would not be obtained with a complex polypeptide mixture such as beer. Furthermore substantial interference, including a high level of background fluorescence, was caused by beer components, especially in ales. Use of the method, after reducing interference by dilution or dialysis of the beer, does not appear to confer any additional value over the direct measurement of total protein in beer using Coomassie Blue-binding methods.
- Subjects :
- Background fluorescence
Chromatography
biology
Cytochrome c
food and beverages
General Medicine
Fluorescence
Analytical Chemistry
Dilution
chemistry.chemical_compound
Biochemistry
chemistry
Catalase
behavior and behavior mechanisms
biology.protein
Bovine serum albumin
Naphthalenesulfonate
Food Science
Total protein
Subjects
Details
- ISSN :
- 03088146
- Volume :
- 75
- Database :
- OpenAIRE
- Journal :
- Food Chemistry
- Accession number :
- edsair.doi...........94c6cd99404ca6734bc9b0ee0444be35