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Ferritin Heavy Chain Stimulates HbS-to-HbF Switching in Erythroid Precursor Cells from Sickle Cell Patients
- Source :
- Blood. 108:790-790
- Publication Year :
- 2006
- Publisher :
- American Society of Hematology, 2006.
-
Abstract
- We have found that ferritin heavy chain (FtH), an antioxidant/stress response/iron-storage protein, localizes to the nucleus in K562 cells and represses the human adult beta-globin promoter in transient assays in primate cells (Broyles et al., PNAS98: 9145, 2001). Since other work indicates FtH is also a gene activator of fetal-globin genes, we hypothesize that FtH is a long-sought developmental hemoglobin (Hb) switching factor and that delivery of FtH to human adult erythroid cell precursors will reverse the phenotype to HbF, offering a phenotypic cure for sickle cell disease (SCD). Chromatin immunoprecipitation (ChIP) assays, antisense treatments, and an FtH transgenic mouse have confirmed that FtH is a globin gene regulatory protein in vivo. With erythroid precursor cells from pediatric SCD patients, under an IRB-approved protocol, we have used a two-phase culture system for in vitro maturation of erythroid cells in the presence of FtH, delivered to the cells as pure protein, as an expression plasmid, or as a priority inducer compound that activates the endogenous FtH gene. HPLC with a PolyCAT A column was used to separate and quantify human Hbs. With each mode of delivery, FtH stimulated a complete switch from HbS to HbF. This result was repeatable in multiple experiments using erythroid precursor cells from three different SCD donors. Fluorescently-labeled recombinant human FtH protein was taken into red cell precursors in culture, suggesting that the purified protein can be directly delivered without gene therapy. This method of producing a phenotypic cure in SCD patients should be easy and inexpensive to deliver in vivo.
- Subjects :
- Regulation of gene expression
Expression vector
biology
Activator (genetics)
Genetic enhancement
Immunology
Cell
Cell Biology
Hematology
Biochemistry
Molecular biology
Ferritin
medicine.anatomical_structure
hemic and lymphatic diseases
medicine
biology.protein
Erythroid Precursor Cells
K562 cells
Subjects
Details
- ISSN :
- 15280020 and 00064971
- Volume :
- 108
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi...........943b3371b3c84e6606822c72ec9dbc13