P263 Analysis of multi-year TRALI testing using luminex solid phase product

Aim Analyze the HLA and MICA antibody detection data from multiple years of screening female blood donors for TRALI mitigation. Methods Over 4000 female blood donors with history of at least 1 pregnancy were screened using One Lambda, Inc. LabScreen Mixed Product. The samples were analyzed using HLA Fusion™ software. Normalized/NBG ratios were used to assign negative or positive results. Previously determined Cutoff Ratios were used: Table 1. Results HLA antibody was detected in over 20 % of the female blood donors that presented for donation. Table 2. Conclusions Significant levels of either HLA Class I or Class II antibody were detected in over 20% of female donors (reporting 1 plus pregnancy). This information is important as blood centers assess the impact of additional testing (i.e. Zika) on recruitment strategy, despite reduced blood product usage. Download : Download high-res image (48KB) Download : Download full-size image Download : Download high-res image (102KB) Download : Download full-size image