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Targeted isolation of sulfur-containing metabolites from Lsr2-deletion mutant strain of Streptomyces roseosporus
- Source :
- RSC Advances. 7:37771-37777
- Publication Year :
- 2017
- Publisher :
- Royal Society of Chemistry (RSC), 2017.
-
Abstract
- Deletion of the Lsr2 gene in Streptomyces roseosporus up-regulated silent gene clusters and produced new secondary metabolites. An ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF-MS/MS) method was used to analyze metabolites of the mutant and wild-type strains, and recognize previously unreported sulfur-containing compounds based on their molecular formulas and fragmentation ions. The targeted isolation of unidentified compounds afforded six new sulfur-containing compounds, pyrismycins A–F (1–6), together with seven known analogues 7–13. Their cytotoxic effects were evaluated using four clinically relevant human cancer cell lines, gastric carcinoma SGC7901, breast carcinoma MDA-MB-231, lung carcinoma A549 and hepatocellular carcinoma HepG2. Compound 7 exhibited the most potent cytotoxicity with IC50 values of 1.7, 5.8 and 6.3 μM against the SGC7901, HepG2 and MDA-MB-231, respectively.
- Subjects :
- 0301 basic medicine
Streptomyces roseosporus
biology
Chemistry
General Chemical Engineering
030106 microbiology
Mutant
General Chemistry
medicine.disease
biology.organism_classification
Molecular biology
03 medical and health sciences
Cell culture
Carcinoma
medicine
Fragmentation (cell biology)
Cytotoxicity
Breast carcinoma
Gene
Subjects
Details
- ISSN :
- 20462069
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- RSC Advances
- Accession number :
- edsair.doi...........929f91ac4a52627023fea727d8d76801