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Dieldrin inhibition of gap junctional intercellular communication in rat glial cells as measured by the fluorescence photobleaching and scrape loading/dye transfer assays*1

Authors :
A. Koestner
James E. Trosko
S. Suter
L. R. Lockwood
M. H. El-Fouly
Source :
Fundamental and Applied Toxicology. 9:785-794
Publication Year :
1987
Publisher :
Elsevier BV, 1987.

Abstract

Application of the fluorescence-recovery after photobleaching (FRAP analysis) technique and scrape loading/dye transfer assay was made to measure the presence of gap junctional communication in primary rat glial cells in vitro in the presence and absence of the neurotoxicant and tumor promoter dieldrin, a chlorinated insecticide. Results demonstrate that primary rat glial cells are able to exhibit gap junctional intercellular communication and that dieldrin at noncytotoxic concentrations can modulate gap junctional communication as early as 10 min after exposure to the chemical and that the effect is reversible after 4 hr recovery from the dieldrin exposure. Both the FRAP analysis and the scrape loading/dye transfer assay have validated the observation that dieldrin inhibits gap junctional communication in other cell types using different techniques to measure gap junction function. These results were interpreted as an indication that inhibition of gap junctional communication might contribute to the cellular mechanism of dieldrin's neurotoxicity.

Details

ISSN :
02720590
Volume :
9
Database :
OpenAIRE
Journal :
Fundamental and Applied Toxicology
Accession number :
edsair.doi...........921073c3488b73d9932899be7ad1c18f
Full Text :
https://doi.org/10.1016/0272-0590(87)90185-0