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Endothelium-Dependent Relaxant Responses to Selective 5-HT1B/1D Receptor Agonists in the Isolated Middle Cerebral Artery of the Rat
- Source :
- Journal of Vascular Research. 40:561-566
- Publication Year :
- 2003
- Publisher :
- S. Karger AG, 2003.
-
Abstract
- The vasomotor effects of triptans in the middle cerebral artery (MCA) of rats were studied using the pressurised arteriography method and in vitro vessel baths. Using the arteriograph, MCAs from Sprague-Dawley rats were mounted on two glass micropipettes, pressurised to 85 mm Hg and luminally perfused. Luminally added 5- hydroxytryptamine (5-HT), sumatriptan and rizatriptan induced maximal dilatations of 22 ± 4, 10 ± 2 and 13 ± 5%, respectively, compared to the resting diameter. The relaxant effect of sumatriptan was blocked by the 5- HT1B/1D receptor selective antagonist GR 55562 (10–6M). The use of Nω-nitro-L-arginine and charybdotoxin revealed that the dilatation involved both nitric oxide and endothelially derived hyperpolarising factor. Thus, the earlier demonstrated expression of 5-HT1B/1D immunoreactivity in the endothelium may well translate into a relaxant response to 5-HT and triptans. Using the vessel bath technique, MCA segments were mounted on two metal wires. The relaxant responses to sumatriptan could not be reproduced using this model; instead, weak contractile responses (6 ± 3% of submaximal contractile capacity) were observed. The difference in observations between the experimental models may be related to the maintenance of shear stress in the arteriograph.
- Subjects :
- Agonist
medicine.medical_specialty
Vascular smooth muscle
integumentary system
Endothelium
Vasomotor
Physiology
business.industry
medicine.drug_class
chemistry.chemical_compound
medicine.anatomical_structure
Endocrinology
chemistry
Internal medicine
medicine.artery
Middle cerebral artery
cardiovascular system
Medicine
Serotonin
Cardiology and Cardiovascular Medicine
Neurotransmitter
business
5-HT receptor
circulatory and respiratory physiology
Subjects
Details
- ISSN :
- 14230135 and 10181172
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Journal of Vascular Research
- Accession number :
- edsair.doi...........91654489f7f1d435f5750dc171324201
- Full Text :
- https://doi.org/10.1159/000075806