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Relationship Between Tumor Necrosis Factor-α Release and Granulocyte and Monocyte Adsorption to Cellulose Acetate Beads

Relationship Between Tumor Necrosis Factor-α Release and Granulocyte and Monocyte Adsorption to Cellulose Acetate Beads

Authors :
Yoshiyuki Ueno
Yasuhiko Abe
Yuko Nishise
Shoichi Nishise
Eiki Nomura
Makoto Yagi
Kazuya Yoshizawa
Daisuke Iwano
Takeshi Sato
Yu Sasaki
Source :
Therapeutic Apheresis and Dialysis. 18:252-257
Publication Year :
2014
Publisher :
Wiley, 2014.

Abstract

Tumor necrosis factor-α, (TNF)-α, a proinflammatory cytokine, is produced by activated granulocytes and monocytes (GMs) and implicated as a major factor in inflammatory bowel disease (IBD) pathogenesis. Reduction of TNF-α should improve IBD pathology. GM adsorptive apheresis (GMA) is an effective therapy for inflammatory disorders including IBD. GM adsorption to cellulose acetate (CA) beads induces anti-inflammatory cytokine release, although these effects on TNF-α release are not clarified. We hypothesized that GMA may inhibit TNF-α release. The aim of the present study was to clarify the effects of GM adsorption to CA beads on TNF-α release in vitro. Peripheral blood was incubated with and without CA beads and TNF-α was measured. For comparison, TNF-α was measured in another lipopolysaccharide (LPS)-containing peripheral blood sample incubated similarly. The amount of TNF-α in blood samples incubated with CA beads was significantly higher than in those incubated without beads, although it was significantly lower than TNF-α incubated with LPS-containing sample without beads. The amount of TNF-α after incubation with CA beads positively correlated with GM adsorption ratio. GM adsorption to CA beads induced a small amount of TNF-α release. This is the first report on TNF-α release induced via GM adsorption stimuli. The biological effects of TNF-α release during GM adsorption need to be clarified.

Details

ISSN :
17449979
Volume :
18
Database :
OpenAIRE
Journal :
Therapeutic Apheresis and Dialysis
Accession number :
edsair.doi...........91515337ede5f24f896b2f7909c382a6
Full Text :
https://doi.org/10.1111/1744-9987.12212