Activité phospholipasique a du suc duodenal humain méthode de détermination par les [32p]phosphatidylcholines

A method has been developed to measure the phospholipase A activity of the duodenal juice; its technical variation coefficient is 5%. This method is based on hydrolysis of [ 32 P]phosphatidylcholine with thin-layer chromatography and determination of the radioactivity of [ 32 P]lysophosphatidylcholine formed without previous lipid extraction. Chromatographic plates radioactivity can be recorded. The peaks are integrated, their surface being proportional to radioactivity.