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Gene-carried chitosan-linked polyethylenimine induced high gene transfection efficiency on dendritic cells

Authors :
Jian-Qing Gao
Gui-Xin Ruan
Xinglei Yao
Guping Tang
Yu-Zhe Chen
Qing-Qing Zhao
Yasuhiko Tabata
Source :
Biotechnology and Applied Biochemistry. 59:346-352
Publication Year :
2012
Publisher :
Wiley, 2012.

Abstract

A dendritic cell (DC) networking system has become an attractive approach in cancer immunotherapy. Successful DC gene engineering depends on the development of transgene vectors. A cationic polymer, chitosan-linked polyethylenimine (PEI) (CP), possessing the advantages of both PEI and chitosan, has been applied in nonviral transfection of DCs. Physicochemical evaluation showed that CP/DNA complexes could form cationic nanoparticles. Compared with DCs transfected with commercial reagent, Lipofectamine2000, it showed higher transfection efficiency and lower cytotoxicity when DCs were transfected with CP/DNA complexes. A nuclear trafficking observation of CP/DNA complexes by a confocal laser scanning microscope further revealed that the CP could help DNA enter into the cytoplasm and finally into the nucleus of a DC. Finally, vaccination of DCs transfected with CP/DNA encoding gp100 slightly improved resistance to the B16BL6 melanoma challenge. This is the first report that CP polymer is used as a nonviral vector for DC gene delivery and DC vaccine. Essentially, these results might be helpful to design a promising nonviral vector for DC gene delivery.

Details

ISSN :
08854513
Volume :
59
Database :
OpenAIRE
Journal :
Biotechnology and Applied Biochemistry
Accession number :
edsair.doi...........9111f76f59c3bb589745bd93bb793ff3