MOESM3 of Chimeric Plasmodium falciparum parasites expressing Plasmodium vivax circumsporozoite protein fail to produce salivary gland sporozoites

Additional file 3. Sequence analysis of the P. vivax csp genes introduced into P. falciparum. Long-range PCR fragments (see Fig. 1) were cloned into pET TOPO TA (Invitrogen) and sequenced. See Additional file 2 for primer sequences used for sequencing the complete fragment. A. LR-PCR fragment of parasites of pf-pvcsp(vk210). B. LR-PCR fragment of parasites of pf-pvcsp(vk247).