Gas Liquid Chromatographic Determination of Mono- and Diglycerides

Method for determination of mono- and diglycerides by gas liquid chromatography as their TMS derivatives or acetates were examined. Chromatograms which show completely separated and sharp peaks were obtained for the both derivatives.Calibration factors for glycerides of fatty acids of carbon numder 14, 16 and 18 were determined by using standard materials. Peak areas observed for diglycerides were relatively small in comparison with those for monoglycerides.Five sorts of commercial products of monoglycerides were used for investigation. Saponification value and hydroxyl value calculated from the GLC composition of the products well agreed with those values determined by chemical analysis, respectively. Contents of monoglyceride of the products determined by GLC and chemical analysis also showed good coincidence.