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New biphasic solvent system based on cyclopentyl methyl ether for the purification of a non-polar synthetic peptide by pH-zone refining centrifugal partition chromatography
- Source :
- Journal of Separation Science. 37:1222-1228
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- A new type 1 ternary biphasic system composed of cyclopentyl methyl ether, dimethylformamide and water was developed, characterized and successfully used for the purification of a lipophilic, protected peptide by pH-zone refining centrifugal partition chromatography. The protected peptide is an 8-mer, key intermediate in bivalirudin (Angiomax®) synthesis and shows a very low solubility in the solvents usually used in liquid chromatography. All ionic groups, except the N-terminal end of the peptide, are protected by a benzyl group. The purification of this peptide was achieved with a purity of about 99.04% and a recovery of 94% using the new ternary biphasic system cyclopentyl methyl ether/dimethylformamide/water (49:40:11, v/v) in the descending pH-zone refining mode with triethylamine (28 mM) as the retainer and methanesulfonic acid (18 mM) as the eluter.
Details
- ISSN :
- 16159306
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Journal of Separation Science
- Accession number :
- edsair.doi...........8ed32400fbf5bf710583c572d71484bb