AAV-Mediated Gene Delivery to the Lung

Authors :: Sarah K. Wootton
Jakob M. Domm
Laura P. van Lieshout
Source :: Methods in Molecular Biology ISBN: 9781493991389
Publication Year :: 2019
Publisher :: Springer New York, 2019.
Abstract: Adeno-associated virus (AAV) vectors are an efficient method of gene delivery to various tissues including the lung. Mouse models are often used as a preliminary preclinical model in order to advance AAV lung gene therapy vectors. In this chapter we describe an AAV purification protocol using heparin affinity chromatography as well as an intranasal and intratracheal method of delivering AAV vectors to the lungs of mice.

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