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Construction of a hydrazine oxidoreductase gene recombinant bacterium for effective N2H4 removal from wastewater
- Source :
- Journal of Water Process Engineering. 44:102366
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Anaerobic ammonium oxidation (anammox) receives considerable attentions from researchers because it could achieve biological denitrification from wastewater with low carbon resource input. During anammox, hydrazine oxidoreductase (HZO) plays a critical role in the biological conversion of ammonium (NH4+) to dinitrogen gas. In this study, we constructed a HZO gene recombinant bacterium by genetic process technology with the aim to effectively remove N2H4 in anammox process. The result indicates about 80% N2H4-N could be removed by the obtained HZO gene recombinant bacterium under the optimum condition (an initial N2H4-N concentration of 5 mg/L and a bacterial dosage of 20 mL). More importantly, the high N2H4-N removal efficiency (about 70%) could be maintained when HZO gene recombinant bacterium spread to the sixth generation. The recombinant protein of HZO gene recombinant bacterium could be successfully expressed and show a stable activity to N2H4 removal under low intensity wastewater, suggesting the removal of N2H4 was attributed to the successful expression of HZO enzyme of anammox bacterium in E. Coli.
- Subjects :
- chemistry.chemical_classification
Denitrification
biology
Chemistry
Process Chemistry and Technology
biology.organism_classification
law.invention
chemistry.chemical_compound
Biochemistry
Wastewater
Anammox
law
Oxidoreductase
Recombinant DNA
Oxidoreductase Gene
Ammonium
Safety, Risk, Reliability and Quality
Waste Management and Disposal
Bacteria
Biotechnology
Subjects
Details
- ISSN :
- 22147144
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Journal of Water Process Engineering
- Accession number :
- edsair.doi...........8c3d6434f166a9c766e86e50b4beb987